WebKLF2 (kruppel-like factor 2 [lung]) regulates osteoclastogenesis by modulating autophagy Macroautophagy/autophagy is involved in myeloid cellular repair, destruction, and osteoclast differentiation; conversely, KLF2 (kruppel-like factor 2 [lung]) regulates myeloid cell activation and differentiation. WebHippo信号通路由一组保守的激酶构成，是一条抑制细胞生长的信号通路。 在哺乳动物中，Hippo信号通路上游的膜蛋白受体感受到胞外环境的生长抑制信号后，经过一系列激酶的磷酸化反应，最终作用于下游效应因子YAP …
MEF2 (Myocyte Enhancer Factor 2) Is Essential for …
WebNov 30, 2024 · KLF2 is a potent anti‐inflammatory transcription factor that maintains normal endothelial function. 5 To determine whether SAHA‐mediated KLF2 upregulation … WebMar 27, 2024 · KLF2-V5– or mock-transduced HUVECs were washed twice with PBS before cross-linking with 37% formaldehyde for 10 min at room temperature. Cells were lysed with cytoplasmic lysis buffer, followed by nuclear lysis buffer. Extracts of nuclei were sonified using Bransons Diagenode for 45 cycles on high amplitude (30 s on followed by 30 s off) … syringe protector
Therapeutic potential of KLF2-induced exosomal microRNAs in
To investigate the effects of shear flow on YAP activity in endothelial cell, we used a HUVEC cell line which is reliable and widely used in studying the mechanism of arteriosclerosis, because HUVEC cells are characterized by unlimited growth while maintaining endothelial cell characteristics16. HUVECs were … See more As UF downregulated the total abundance of YAP, we subsequently wanted to ascertain how YAP is inactivated under UF. A previous study revealed that YAP can be inactivated by … See more We subsequently examined autophagy levels in HUVECs by monitoring LC3 puncta with immunofluorescence. LC3 staining was relatively weaker in CD31-positive ECs treated with DF compared with those exposed to … See more Post-translational modifications, such as phosphorylation, acetylation, and ubiquitination, define the transcriptional activation of YAP. In … See more To ascertain whether autophagy inhibits the development of atherosclerosis by manipulating YAP, we treated HUVECs with a … See more WebJun 1, 2006 · The flow-responsive transcription factor KLF2 is acquiring a leading role in the regulation of endothelial cell gene expression. A genome-wide microarray expression profiling is described employing lentivirus-mediated, 7-day overexpression of human KLF2 at levels observed under prolonged flow. WebKLF2: A gene on chromosome 19p13.11 that encodes a member of the Kruppel-like family of broadly expressed zinc finger transcription factors, which regulates T-cell trafficking by … syringe procedure