Fischer rat thyroid frt cells

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August undefined, 2024

WebFischer Rat Thyroid 5 Epithelial Cell Line Frt, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … WebJan 12, 2024 · Fisher rat thyroid (FRT) cells were cultured in DMEM/F-12 medium (plus 8% FBS) and transiently transfected with plasmids (0.5 µg per construct) using Lipofectamine 3000 (Invitrogen, L3000008) according to the manufacturer’s instruction. An equal amount of a mouse PON2 cDNA or the pCMV6 vector was cotransfected with …

Elexacaftor is a CFTR potentiator and acts synergistically with ...

WebJul 16, 2024 · Cell culture. Fischer Rat Thyroid (FRT) cells: Isogenic FRT cells were stably transduced using the Flp-In™ System (Thermo Fisher Scientific, Inc.) with either a … WebA, total RNA was isolated from Fisher rat thyroid (FRT) cells and RT-PCR was performed to identify endogenous expression of ANO proteins. FRT cells expressed mRNA for ANO6, ANO8, and ANO10, whereas no … cinder blocks wikipedia

Activation of second messenger-dependent protein

WebSep 8, 2009 · functional and biochemical assays on two different cell expression systems, Fischer rat thyroid (FRT) and A549 cells. The order of potency and efﬁcacy of … WebJul 26, 2024 · Screening was done using a Fischer rat thyroid (FRT) cell line stably expressing murine slc26a3 and a halide-sensitive yellow fluorescent protein (FRT-YFP-slc26a3 cells). FRT cells were used because of their low intrinsic permeability to anions that are transported by slc26a3 and because of their good adherence and rapid growth on … WebFRT are poorly differentiated epithelial cell line derived from Fischer rat thyroid gland. FRT cells are well polarized both morphologically and functionally and among the thyroid markers expresse only Pax8 a … diabetes and vitamin c

Alteration of Membrane Cholesterol Content Plays a Key …

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WebMar 5, 2001 · Fischer rat thyroid (FRT) cells (Lipardi et al. 1998), the gift of Dr. Enrique Rodriguez-Boulan (Cornell University, Ithaca, New York), were maintained in Coon's F12 media with 10% FBS. COS, CHO, and HEK 293 cells (American Type Culture Collection) were maintained in DME with 5% iron-supplemented calf serum or 10% FBS. WebJun 7, 2024 · Treatment with methyl-β-cyclodextrin (MβCD) reduces total cholesterol level in Fischer rat thyroid (FRT) cells. (A) A representative extracted ion chromatogram for m/z 369.3513 (cholesterol) from mass … cinder block under water heaterWebMay 1, 2012 · In this set of in vitro studies, the ability of ivacaftor to potentiate mutant CFTR was examined in a panel of Fischer rat thyroid (FRT) cells engineered to express … diabetes and vision loss statistics

"WebOct 13, 2024 · Fischer rat thyroid (FRT) cells are the only known epithelial cells that sort most of their endogenous GPI-anchored proteins to the basolateral surface (Zurzolo et al., 1993). Although they have TX … " - Fischer rat thyroid frt cells

Fischer rat thyroid frt cells

American Journal of Physiology-Cell Physiology: Vol 322, No 1

WebMay 18, 2024 · Methods: With Fischer rat thyroid (FRT) cells heterologously expressing wild-type and F508del-CFTR and fully-differentiated CF and non-CF human airway epithelial cells, we studied SLC34A2 expression and the effects of phosphate on CFTR-mediated transepithelial ion transport. F508del-CFTR was traﬃcked to WebMay 21, 2024 · Fisher Rat Thyroid (FRT) cells stably transfected with a wild type (WT) CFTR, or CFTR carrying the cystic fibrosis mutation p.F508del, were grown at 37 °C and 5% CO 2 in modified F12 Coon’s ...

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WebJan 12, 2024 · Therefore, to investigate the mechanism by which PON2 regulates ENaC, we expressed PON2 along with the ENaC subunits in fisher rat thyroid (FRT) cells, a system that is amenable to biochemical analyses of ENaC assembly and trafficking. We found that PON2 primarily resides in the endoplasmic reticulum (ER) in FRT cells, and its … WebOct 13, 2024 · In contrast to Madin–Darby canine kidney cells, Fischer rat thyroid cells deliver the majority of endogenous glycosylphosphatidyl inositol (GPI)–anchored proteins …

WebMar 5, 2010 · A, total RNA was isolated from Fisher rat thyroid (FRT) cells and RT-PCR was performed to identify endogenous expression of ANO proteins. FRT cells expressed mRNA for ANO6, ANO8, and ANO10, whereas no detectable signal was found for ANO1, ANO2, ANO3, ANO4, ANO5, ANO7, and ANO9. ± indicates presence or absence of … WebSep 8, 2009 · functional and biochemical assays on two different cell expression systems, Fischer rat thyroid (FRT) and A549 cells. The order of potency and efﬁcacy of potentiators was similar in the two cell types considered, with phenylglycine PG-01 and isoxazole UCCF-152 be-ing the most potent and least potent, respectively. Most …

WebFischer rat thyroid (FRT) cells were stably transfected with a dual-luciferase complementary DNA (cDNA) containing a UGAC or CFTR-G542X readthrough sequence (termed “G542X context”) as shown in Figure 1.For functional assays, cDNAs carrying CFTR-G542X, CFTR-W1282X, or CFTR-WT alleles were introduced into FRT cells …

WebNov 29, 2024 · After extensive experiments, we found that the M3 receptor is endogenously expressed in Fischer rat thyroid (FRT) cells. FRT cells can be grown on relatively inexpensive media with good adhesion to commonly used cell culture plates while allowing stable structures expression of the transfected protein. Therefore, we applied FRT cells … diabetes and vaginal itchWeb5 rows · Rat: Fisher rat thyroid cells: Tissues: Thyroid: Phenotype: Suspension: Primary: no: ... diabetes and tooth pain diabetes and urinary infectionsWebThirty novel 5-substituted benzyloxy-2-arylbenzofuran-3-carboxylic acids (B01-B30) were synthesized and evaluated for their TMEM16A inhibitory activity by using short circuit current measurements in Fischer rat thyroid (FRT) cells expressing human TMEM16A. IC(50) values were calculated using YFP fluorescence plate reader assay. cinder blocks window sillWebAug 31, 2004 · In primary cultures of dog thyroid cells and in non-differentiated Fischer rat thyroid (FRT) epithelial cells, mAChR stimulation induces a transient increase in the intracellular free Ca 2+ mobilization via phospholipase C (PLC) activation, and Ca 2+ store depletion, capacitative Ca 2+ entry and voltage-dependent Ca 2+ channel activation … cinder block top and bottomWebJan 7, 2010 · Fisher rat thyroid (FRT) cells stably expressing human CFTR were used to study CFTR function as they have negligible expression of non-CFTR chloride channels . In addition, they can form epithelial monolayers on Snapwell filters with electrical resistance high enough for use in electrophysiological studies. Our data showed that in basolaterally ... diabetes and vision issuesWebIn striking contrast with these model polarized cell lines, we show here that Fischer rat thyroid (FRT) epithelial cells do not display a preferential apical distribution of GPI … cinder blocks with holes